Effect of acute hypobaric hypoxia at high altitude on the expression of hemoglobin and miR-144 in plasma in rats

doi:10.3877/cma.j.issn.2095-6568.2018.04.002

Abstract

Abstract:

Objective

This study investigated the effect of acute hypobaric hypoxia at high altitude on the expression of hemoglobin and miR-144 in plasma in rats.

Methods

Adult male Sprague-Dawley rats were randomly assigned to a normoxic group and four hypoxic groups. Rats in hypoxic groups were exposed to simulated hypobaric hypoxia chamber equivalent to 7 000 meters for different durations (3, 7, 14 and 28 days). Subsequently, blood routine test and the expression of miR-144 in plasma were measured in each group.

Results

Compared with the normoxic group, the expression of miR-144, erythrocyte count and hemoglobin in four hypoxic groups increased significantly (P<0.05). Spearman correlation analysis showed that the expression of miR-144 in plasma was positively correlated with erythrocyte count and hemoglobin.

Conclusion

Acute hypobaric hypoxia at high altitude increased the expression of miR-144 in plasma and hemoglobin. It is predicted that miR-144 in plasma could induce polycythemia related to hypobaric hypoxia at high altitude. Regulations on miR-144 expression had a great value in clinical application.

Key words: High altitude, Hypobaric hypoxia, miR-144, Polycythemia

Zhenglong Feng, Bing Shi, Yongqi Zhao, Lingling Zhu, Junxia Li. Effect of acute hypobaric hypoxia at high altitude on the expression of hemoglobin and miR-144 in plasma in rats[J]. Chinese Journal of Heart and Heart Rhythm(Electronic Edition), 2018, 06(04): 195-198.

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URL: https://zhxzyxldzzz.cma-cmc.com.cn/EN/10.3877/cma.j.issn.2095-6568.2018.04.002

https://zhxzyxldzzz.cma-cmc.com.cn/EN/Y2018/V06/I04/195

组别	例数	RBC(×10¹²/L)	HGB(g/L)	RDW(%)
对照组	6	6.60±0.40	131.33±6.24	13.88±0.58
低氧3 d组	9	8.29±0.38	176.00±4.74	24.41±1.68
低氧7 d组	9	8.99±0.47	183.67±8.30	26.69±1.65
低氧14 d组	9	9.43±0.67	211.89±16.83	29.93±1.60
低氧28 d组	9	10.74±0.69	242.00±7.02	30.16±1.60

组别	例数	RBC(×10¹²/L)	HGB(g/L)	RDW(%)
对照组	6	6.60±0.40	131.33±6.24	13.88±0.58
低氧3 d组	9	8.29±0.38	176.00±4.74	24.41±1.68
低氧7 d组	9	8.99±0.47	183.67±8.30	26.69±1.65
低氧14 d组	9	9.43±0.67	211.89±16.83	29.93±1.60
低氧28 d组	9	10.74±0.69	242.00±7.02	30.16±1.60

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